Double stranded DNA templates for the electrochemical determination of benzene derivatives
نویسنده
چکیده
Multi-walled carbon nanotubes (MWCNT) modified DNA based biosensors were prepared by immobilizing dsDNA over MWCNT coated graphite electrode. The film forming abilities of MWCNT coated graphite electrodes were studied using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) by placing the electrodes in electrolyte containing redox couple. Differential Pulse Voltammetry (DPV) and EIS were performed to identify the relative change in the oxidation peak and charge transfer resistance respectively after its interaction with benzene derivatives (aniline, toluene, phenol, anisole and mesitylene). Under optimized conditions, calibration curves were obtained for the modified electrodes over various analytes using DPV and EIS. The proposed ds-DNA based MWCNT biosensor exhibited stability, high reproducibility, selectivity and regeneration, making it a potential tool for electrochemical detection of benzene derivatives in water based samples.
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